Cytological analysis of meiosis in Caenorhabditis elegans.
نویسندگان
چکیده
The nematode Caenorhabditis elegans has emerged as an informative experimental system for analysis of meiosis, in large part because of the advantageous physical organization of meiotic nuclei as a gradient of stages within the germline. Here we provide tools for detailed observational studies of cells within the worm gonad, including techniques for light and electron microscopy.
منابع مشابه
Spermatogenesis-Specific Features of the Meiotic Program in Caenorhabditis elegans
In most sexually reproducing organisms, the fundamental process of meiosis is implemented concurrently with two differentiation programs that occur at different rates and generate distinct cell types, sperm and oocytes. However, little is known about how the meiotic program is influenced by such contrasting developmental programs. Here we present a detailed timeline of late meiotic prophase dur...
متن کاملCrossing over during Caenorhabditis elegans meiosis requires a conserved MutS-based pathway that is partially dispensable in budding yeast.
Formation of crossovers between homologous chromosomes during Caenorhabditis elegans meiosis requires the him-14 gene. Loss of him-14 function severely reduces crossing over, resulting in lack of chiasmata between homologs and consequent missegregation. Cytological analysis showing that homologs are paired and aligned in him-14 pachytene nuclei, together with temperature-shift experiments showi...
متن کاملChromosome-wide regulation of meiotic crossover formation in Caenorhabditis elegans requires properly assembled chromosome axes.
Most sexually reproducing organisms depend on the regulated formation of crossovers, and the consequent chiasmata, to accomplish successful segregation of homologous chromosomes at the meiosis I division. A robust, chromosome-wide crossover control system limits chromosome pairs to one crossover in most meioses in the nematode Caenorhabditis elegans; this system has been proposed to rely on str...
متن کاملSNP-based mapping of crossover recombination in Caenorhabditis elegans.
Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. Here, we provide methods for the use of single-nucleotide polymorphisms (SNPs) for the study of crossing over in C. elegans.
متن کاملExposure to Oil during Meiosis Results in Alterations in Meiotic Chromosomes that are Similar to Age-Related Changes in the Nematode Caenorhabditis elegans
Exposure of young C. elegans nematodes to three different concentrations of oil resulted in changes in the meiotic chromosomes, nucleus, nucleolus, and nuclear envelope associations. Such alterations decreased the viability and fertility of this organism which was used as a biological model. The morphological changes in the “young” group were similar to nematodes that were senescent and post-re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Methods in molecular biology
دوره 558 شماره
صفحات -
تاریخ انتشار 2009